Abstract: The Ningwu and Luzong basins in the Yangtze River region are characterized by the occurrence of extensive iron ore deposits. The igneous rocks associated with iron deposits in the two basins share similar petrologic features, although they differ in geochemical and isotopic signatures. To further reveal the differences of ore-bearing rocks in two basins, this paper conducted comparative study on petrography, major and trace elements, rare earth elements, Sr-Nd and zircon Hf isotopic compositions of the main ore-bearing igneous rocks from the two basins. The observation results under microscope show that two basins have the same mineral assemblages. Analyses of major elements indicate that the rocks are characterized by medium Si,and high K and Mg, suggestive of intermediate-mafic igneous rocks. Compared with the ore-bearing strata in the Ningwu basin, the strata in the Luzong basin contain relatively high ∑REE, ∑LREE and ∑HREE, especially with enrichment in Th and U and obvious negative Eu anomaly, suggesting that the ore-bearing rocks of the Luzhong basin contain much crust material. The Sr-Nd isotopic compositions indicate that the igneous rocks in the two basins were derived from enriched mantle. The lower zircon Hf isotopic compositions of the Luzong basin suggest that during the process of intrusion and evolution of magma, more crustal materials were added and this process resulted in the reduction of magma viscosity. Therefore it was more favorable for the iron mineralization in the Luzong basin.