Abstract: According to the characteristics of resources and environment in Mudanjiang City, four factors, i.e. land resources, water resources, environment and hazard are selected as evaluation indexes to establish a three-level evaluation index system of resources and environment carrying capacity in Mudanjiang City. In virtue of GIS spatial analysis technology, the carrying capacity of resources and environment in Mudanjiang City was evaluated through integrated evaluation of agricultural and urban function direction on the basis of the single evaluation of each factor. The results show that the areas with higher agricultural production carrying capacity are mainly distributed in Ning'an City and Dongning City, accounting for 48.87% of the total area, and the areas with higher urban construction carrying capacity are mainly distributed in the north of Ning'an City, the south of Hailin City and the urban area, accounting for 44.99%, 68.34% of the current cultivated land has higher agricultural production carrying capacity and 79.73% of the construction land has higher urban construction carrying capacity. The evaluation results are in line with the reality of Mudanjiang City, and can provide reference for resource development and utilization, ecological environment protection and land space planning of Mudanjiang City.