Abstract: In order to show the application effect of comprehensive geophysical survey in gold polymetallic ore exploration in Bengbu uplift area and better guide the prospecting work, this paper carried out areal high-precision gravity-magnetic survey and comprehensive geophysical profile survey in the Huaiyuan Shuanggou case study area, and then preliminarily built a superimposed structural framework of near EW and NE-near NS direction. Based on drilling verification, two types of gold polymetallic mineralization zones respectively controlled by ductile-brittle and brittle fractures are discovered. Accordingly, two kinds of gold polymetallic ore metallogenic favorable areas are concluded, namely the NE, near NS magnetic anomaly center, and the NE, near NS gravity-magnetic anomaly cascade zone. The NE, near NS magnetic anomaly, the NE, near NS gravity-magnetic anomaly cascade zone, local gravity height under the background of gravity anomaly cascade zone, and the apparent resistivity contour cascade zone or obvious fault zone on the CSAMT profile are recognized as the comprehensive geophysical prospecting indicators for the gold polymetallic ore in the region.